888.3net新浦京游戏(国际)官网-Best Gaming Platform

Product News
Home > Company > News > Product News > From PD to Manufacturing: Study on Consistency during Scaling-up from DuoBioX Explore to Pro Bioreactors

From PD to Manufacturing: Study on Consistency during Scaling-up from DuoBioX Explore to Pro Bioreactors

Sep. 26, 2023

At Duoning, we provide DuoBioXTM series cell culture bioreactors from process development to large scale manufacturing. The Explore series of benchtop bioreactors are positioned for upstream process development, optimization, and characterization at the laboratory stage, with volume ranging from 500 mL-15 L. The Pro series single-use bioreactors are production systems that meet the GMP requirements of commercial scale, with volume ranging from 50 to 2,000 L.


The Explore bioreactors are benchtop glass bioreactors with a distributed layout of satellite tanks, using centralized control, capable of supporting 0.6 - 15 L culture volumes. The software adopts advanced SCADA architecture, which can realize multi-parallel culture operation, data acquisition and result comparative analysis.

From PD to Manufacturing: Study on Consistency during Scaling-up from DuoBioXTM Explore to Pro Bioreactors

DuoBioXTM Explore Parallel Bioreactors


Pro series single-use bioreactors adopt bottom stirring tank design, which can realize continuous scale-up from 50 L - 2,000 L. Combined with 3D single-use cell culture bags developed and manufactured by Duning, which feature good biocompatibility, and flexible manifold design, can meet all kinds of complex requirements in upstream processes. Pro bioreactors' mixing and sparging integrated design not only provides good mixing effect but also ensures excellent gas mass transfer performance.

From PD to Manufacturing: Study on Consistency during Scaling-up from DuoBioXTM Explore to Pro Bioreactors 

DuoBioXTM Pro Single-use Bioreactors


Scale-up consistency of cell culture process - For benchtop bioreactors in the process development stage, it is particularly important to provide reliable process parameters for subsequent scale-up, so 3 L – 5 L bioreactors are usually used as the starting point for the development of the cell culture process and as a tool for continuous optimization. In this study, the basic scaling principle of constant power consumption per unit volume (P/V) was used to investigate the consistency of the culture process from the Explore benchtop 3 L to the Pro 500 L, and the excellent scaling consistency between the two scales was proved.


CHO Cell Line


The cell line used in this study was recombinant CHO-K1 cell, and the product expressed by the cells was a bispecific antibody. CHO (Chinese Hamster Ovary) cells are the most representative mammalian expression hosts for genetically engineered proteins, and they are the most successful cells for expressing foreign proteins. CHO cells were able to grow to high density in serum-free suspension culture and maintain high levels of protein expression during extended cell culture.


CHO cells are the preferred manufacturing hosts for more than 70% of protein-based therapeutics, including monoclonal antibodies, recombinant proteins, and vaccines. At present, the commonly used CHO cell lines are: CHO-K1, CHO-DXB11, CHO-S, CHO-DG44, CHO-GS, etc.




Cell culture media

Basal media: Media C (also for seed train)

Feed media: DN feed 2, DN feed B2


Cell strain



Bioreactor systems

DuoBioXTM Explore multi-parallel bioreactor, 3 L

DuoBioXTM Pro single-use bioreactor, 200 L & 500 L




Cell culture


For fed-batch mode, the initial culture volume is generally less than the maximum working volume of the tank, and the space left will be intermittently replenished several times during the culture process, so the culture volume will gradually increase until the end of the culture. After the cells are seeded, they consume nutrients (e.g., carbon sources, nitrogen sources) from the initial media for growth. With the continuous increase of cell density, the nutrients in the tank will be depleted, while the metabolic wastes continue to accumulate, but the nutrients required by the cell growth can be supplemented in time by feeding, so that the cell density will further increase. When the metabolic wastes accumulate to the point that the cell growth is significantly affected, the cell density and viability will also start to drop from the peak value.


pH control


pH down-regulation was performed by adding CO2 into the culture through 1 mm sparge to lower pH.


pH up-regulation was performed by pumping base solutions such as Na2CO3, NaHCO3 or NaOH into the culture through a peristaltic pump.


In general, the appropriate pH range for CHO cell culture is 7.0-7.2. In this study, the pH value of the culture was measured online through the pH sensor, and the flow rate of the CO2 mass flowmeter and the speed of the base adding pump were automatically adjusted according to the measured value. The pH control dead zone was set to ±0.2, which could effectively preair supply pH adjustment overshoot.


DO control


The dissolved oxygen control of the culture system is realized by sparging different kinds of gases into the tank. Air and O2 are sparged into the tank to increase DO, while N2 can be used to control DO downregulation, achieving lower DO levels and even maintaining an oxygen-free environment. Due to the low solubility of oxygen in the media, its mass transfer efficiency is affected by the specific surface area of the bottom air supply and the contact time. The suitable range of dissolved oxygen for most mammalian cells is usually between 20% and 60%. The dissolved oxygen in the media is monitored online by an optical DO sensor, while the MFC-O2 flow rate is automatically adjusted according to the measured value.


In this study, the pore size of the sparger at the bottom of the Explore benchtop bioreactor was 1 mm, and the blade was a classic single-layer three-blade type with angle of 45°, which could generate downward axial flow and reduce the shear force on cells due to agitation. At the initial stage of the culture of Pro single-use bioreactor, 1mm macro-sparger bottom air supply was adopted, and the strategy was to combine macro-sparger and micro-sparger. When the VVM reached 0.3, turn on the 20 µm bottom micro-sparger as additional.


Feed control


Due to the fed batch operation mode adopted, regular feeding was required during the culture process. For the Explore benchtop bioreactor, the auxiliary simulation 4-20 mA input interface of the controller is used to connect the external scale, and for Pro bioreactors, they came with load cells, so for both bioreactor series, automatic feeding can be performed by built-in Feed In peristaltic pumps controlled by weight, and the feeding time point and frequency are programmed in the control software to run automatically.


Parameter setting and control strategy – fed-batch

Process parameters




Working volume

1 L

160 L

460 L

Seeding density

1.5E6 cells/mL

Culture duration

16 days


36.5 ℃, and decrease to 33 ℃ on day 4






Basal: Media C, Feed: DN feed 2 & DN feed B2, all from Duoning

Feed strategy

3% DN feed 2+ 0.3% DN feed B2 from day 3




Bioreactor configuration





Temp. sensor


pH sensor

Chemical gel electrode (Mettler Toledo)

DO sensor

Optical digital electrode (Hamilton)

Bottom air supply

1mm macro-sparger, 7 holes layout as “L”

1mm macro-sparger, 20μm micro-sparger

1mm macro-sparger, 20μm micro-sparger

Overlay air supply

Rotor flow meter 0-1000 mLPM



Peristaltic pump

4 x Watson-Malow 114D

2 x Watson Marlow 114D

2 x Watson Marlow 313D

2 x Watson Marlow 114D

2 x Watson Marlow 313D


Semiconductor Cooling (Peltier)

Electrical heating

Electrical heating






Tank body and temp. control

Glass, electric blanket   heating

Stainless steel water jacket

Max. working volume

2 L

200 L

500 L

Min. working volume

0.6 L

40 L

100 L


Three inclined blades

Three inclined blades, bottom stir




Aspect ratio





28 W/m3


Viable cell density and viability

From PD to Manufacturing: Study on Consistency during Scaling-up from DuoBioXTM Explore to Pro Bioreactors


Cell metabolism and protein concentration

From PD to Manufacturing: Study on Consistency during Scaling-up from DuoBioX Explore to Pro Bioreactors

From PD to Manufacturing: Study on Consistency during Scaling-up from DuoBioX Explore to Pro Bioreactors



According to the basic scaling principle of constant P/V, the fed batch culture process of CHO-K1 cells was successfully scaled up from a 3 L benchtop bioreactor to 200 L and 500 L single-use bioreactors, and the cell growth performance and protein productivity (>4 g/L) showed good consistency.


Information of related products


Cell culture media

Product No.

Product Name



Media C

1 L liquid


Media C

100 g dry powder


Media C

1 kg dry powder


Media C

10 kg dry powder


DN feed 2

1 L liquid


DN feed 2

100 g dry powder


DN feed 2

1 kg dry powder


DN feed 2

5 kg dry powder


DN feed B2

300 mL liquid


DN feed B2

100 g dry powder



Product No.

Product Name


Explore 3 L bioreactor system, single tank


Explore 3 L bioreactor system, 4 tanks in parallel


Explore 3 L bioreactor system, 8 tanks in parallel


Pro-50L Single-use bioreactor


Pro-200L Single-use bioreactor


Pro-500L Single-use bioreactor


Pro-1000L Single-use bioreactor


Pro-2000L Single-use bioreactor


Related Products
Contact Us
  • Tel.: +86 021 6434 0155
  • E-Mail: marketing@duoningbio.com
  • Address: Building 30, No. 1525 Minqiang Road, Songjiang District, Shanghai

Copyright © Shanghai Duoning Biotechnology Co., Ltd. All Rights Reserved Sitemap | Technical Support: Reanod



Duoning Biotechnology

+86 021 6434 0155

XML 地图